Poly L Lysine Coated Glass Bottom Dish

Eliminate cell detachment and background noise in live-cell imaging. Our precision-engineered glass bottom dishes provide the ideal electrostatic environment for demanding primary cells and neurons, designed specifically for Technical Directors and Lab Managers seeking
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Optimize Cell Attachment: Poly L-Lysine Coated Glass Bottom Dishes for High-Resolution Imaging—Request Free Samples Today

Eliminate cell detachment and background noise in live-cell imaging. Our precision-engineered glass bottom dishes provide the ideal electrostatic environment for demanding primary cells and neurons, designed specifically for Technical Directors and Lab Managers seeking ROI through reproducible data.

Why Standard Glass Bottom Dishes Fail Your Research

In the world of high-resolution microscopy, the interface between the cell and the substrate is where most experiments fail. We've spoken with hundreds of lab managers who struggle with the same recurring issues:

1. Poor Cell Adhesion: Non-polar glass surfaces often lead to cell "sloughing" or detachment during media changes, ruining weeks of culture.
2. Inconsistent Coating: Patchy Poly L-Lysine (PLL) application creates "dead zones," leading to non-uniform cell distribution and skewed data.
3. High Procurement Costs: Over-reliance on a few Western brands leads to inflated pricing and excessive shipping lead times that stall project timelines.
4. Optical Aberrations: Low-grade glass thickness variations cause spherical aberration, blurring the image at the 40x or 60x oil immersion level.

Stop compromising your ROI with inconsistent consumables. Your data is only as good as your substrate.

The SHAHE SHOTT Engineering Advantage

Our poly L lysine coated glass bottom dish isn't just a lab consumable; it's a precision-engineered optical tool. By leveraging 28 years of glass fabricating expertise, we integrate medical-grade ultra-clear glass with a proprietary covalent bonding process for the PLL coating.

  • Ultra-High Transparency: Utilizing ultra-clear float glass to minimize auto-fluorescence.
  • Uniform Positive Charge: Our PLL coating ensures a consistent positive surface charge, maximizing the electrostatic attraction of negatively charged cell membranes.
  • Precision Thickness: Controlled to within ±0.05mm to ensure compatibility with high-NA objectives.
  • OEM/ODM Flexibility: Custom diameters and coating densities available to meet specific cell-line requirements.

Technical Specifications Table

Parameter Standard Specification Customizable Range
Glass Material Borosilicate / Ultra-Clear Float Custom Grade Available
Coating Material Poly-L-Lysine (PLL) Poly-D-Lysine / Collagen / Laminin
Bottom Thickness 0.17mm (Standard) 0.1mm - 1.0mm
Dish Diameter 35mm / 50mm Custom Molded Sizes
Sterilization Gamma Irradiation / ETO Client-specified
Surface Roughness Ra < 10nm Super-polished options

Application Scenarios

Neuroscience Research: Ideal for primary hippocampal or cortical neurons which require strong adhesion to form complex dendritic networks.
Live-Cell Confocal Imaging: The 0.17mm glass thickness allows for high-NA oil immersion lenses to capture Z-stack images with minimal aberration.
Drug Toxicity Screening: Uniform coating ensures that cell death is a result of the compound, not inconsistent surface attachment.

PLL-coated-glass-slides-for-HEK fluorine-doped-tin-oxide-coated-glass-slide ITO-coated-glass-slide gold-coated-glass-slide

Trusted by Global Biotech & Research Institutions

Siemens GE IBM Shell
Customer Dr. Elena Rossi, Senior Researcher

"We switched to SHAHE SHOTT's PLL coated dishes for our neuronal culture. The attachment rate increased by 22% compared to our previous US supplier, and the cost per unit dropped by nearly 30%."

Customer Mark Thompson, Lab Ops Manager

"The delivery speed was the game-changer. We received our custom 50mm batch in 14 days. The optical clarity is flawless, meeting all our confocal microscopy requirements."

Certified Quality Standards

ISO 9001 CE FCC RoHS

Frequently Asked Questions

Q: How does the Poly L-Lysine coating affect the pH of the medium?

A: Our coating process involves a rigorous rinsing cycle to remove unbound PLL. This ensures that the coating is covalently bonded to the glass, resulting in negligible impact on the pH of your culture medium.

Fto Coated Glass Substrate

Q: Can you provide OEM designs for specific dish geometries?

A: Yes. With our Intermac CNC work center and precision cutting tables, we can manufacture glass bottoms to your exact blueprints, including non-circular shapes or varied thickness profiles.

Q: What is the shelf life of the PLL coating?

A: When stored in the provided vacuum-sealed, moisture-proof packaging at 4°C, our coated dishes maintain peak performance for 12 months.

Q: Do you offer bulk pricing for long-term supply contracts?

A: Absolutely. We specialize in wholesale trade for biotech distributors. We offer tiered pricing based on volume and can establish "Safety Stock" agreements to ensure 0-day lead times for repeat orders.

Q: How do you handle international shipping to avoid breakage?

A: We use customized EPE foam inserts and double-walled corrugated boxes. Our shipping protocols are designed for the fragile nature of precision glass, with a guaranteed replacement policy for any items damaged in transit.

Ready to Elevate Your Imaging Resolution?

Don't let poor cell adhesion compromise your next publication. Join 500+ global labs using SHAHE SHOTT precision glass.

Limited Offer: Free Sample Kit for New Labs this Month!

Risk-Free Guarantee: If the adhesion rate doesn't meet your specifications, we offer a full refund or free re-coating.

Voices from the Bench

Reviewer "The consistency of the PLL coating is what impressed me most. In 50 dishes, we saw zero variance in cell density across the bottom. Truly industrial grade."
- Sarah J., PhD, Boston Biotech
Reviewer "Finally a supplier that understands the difference between 'glass' and 'optical grade substrate'. The Z-axis clarity is exceptional."
- Dr. Hans Müller, Munich Research Center
Reviewer "The OEM service helped us design a specific dish size for our automated imaging rig. The communication was professional and the delivery was on time."
- Kevin L., Ops Director, Singapore BioHub
Expert Author
Written by: James Sterling
Chief Technical Consultant at SHAHE SHOTT Glass Co., Ltd. With over 20 years of experience in precision glass fabrication and optical coatings, James specializes in bridging the gap between industrial manufacturing and laboratory requirements. He has consulted on over 150 custom glass projects for the life sciences sector across Europe and North America.

Industry Perspective & Market Data

The demand for high-precision processed glass is surging. According to the Flat Glass Market Report 2025-2030, coated and value-added glass segments are growing rapidly due to the integration of advanced materials in medical and solar applications. Furthermore, the Global Flat Glass Market analysis highlights that the shift toward specialty glasses—including those used in labware—is driven by stringent safety and performance standards. At SHAHE SHOTT, we align our production with these global trends, ensuring our poly L lysine coated glass bottom dish meets the 2026 standards for transparency and bio-compatibility.

Eliminate cell detachment and background noise in live-cell imaging. Our precision-engineered glass bottom dishes provide the ideal electrostatic environment for demanding primary cells and neurons, designed specifically for Technical Directors and Lab Managers seeking

If you are interested in our products, you can choose to leave your information here, and we will be in touch with you shortly.


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